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Precautions for freezing cells in liquid nitrogen tank

By:adminauthor:admin Date:2022-06-20 15:42 View:

Precautions for freezing cells in liquid nitrogen tank
The liquid nitrogen tank is a low-temperature container. There is a vacuum layer between the inner and outer bladders of the entire container, which can maintain cells, vaccines, semen, tissues and other organisms. It can be stored in the liquid nitrogen tank to maintain activity for a long time, but

when freezing cells, it should be Pay attention to what problems?
First of all, we need to know that when freezing cells, we need to include preparation, sampling, culture and cryopreservation recovery. In order to preserve cells, we need to pay attention to the details of the operation.

Precautions for freezing biological samples in liquid nitrogen tanks:
1: When we use DMSO, we cannot use autoclaving because it has a sterilizing effect. If it is autoclaved again, it will destroy the molecular structure and reduce the cryoprotective effect. Wear gloves as much as possible during the operation to prevent harm to the human body.
2: When we freeze cells, the temperature is between 0 °C and -60 °C, and the time cannot be too long, otherwise it will affect the activity of the cells.
3: Excess freezing solution can be stored at 4 degrees Celsius.
4: During the process of freezing cells, it is necessary to regularly check the remaining amount of liquid nitrogen to prevent the phenomenon of running out of liquid nitrogen.
Disinfection work:

The used glassware needs to be soaked in clean water for 30 minutes, then need to add water again, wash it in ultrasonic for 30 minutes, take it out to dry, then soak it in chromic acid for 6-18 hours, rinse it with water again, Use double-distilled water to wash 3-4 times, after drying and disinfection,

it can be used normally.